Journal: International Journal of Molecular Sciences
Article Title: Expression of Lumican and Osteopontin in Perivascular Areas of the Glioblastoma Peritumoral Niche and Its Value for Prognosis
doi: 10.3390/ijms26010192
Figure Lengend Snippet: CMA activity in PCs correlates with patient survival. ( A ) Representative images of peritumoral areas according to patient classification showing co-localization of puncta pattern expression of LAMP-2A protein (dark brown) with the PC marker α-SMA (pink) in microvessels of GB patients. Samples were classified as severe (highest α-SMA/LAMP-2A co-localization), moderate or mild (basal co-localization) according to the histological evaluation. A1 to A3 shows magnifications of microvessels (v). LAMP-2A co-localizationwith α-SMA + cells is marked with arrows. Scale bar: 100 µm. ( B ) Representative images of PCs marked with PDGFRβ (in red) in microvessels (v) showing co-localization of puncta pattern expression of LAMP-2A protein (in green) in peritumoral areas of severity classified GB patients. B1 to B3 show magnifications of PDGFRβ + cells. Positive co-localization (in yellow) is marked with arrows. Nuclei were stained with DAPI (blue). Scale bar: 100 µm. ( C ) Overall survival shown by Kaplan–Meier curves of the severity classification related to CMA activity in PCs; * p < 0.05: difference between mild and severe; # p < 0.05: difference between mild and moderate. ( D ) Age distribution by gender of the cohort of GB patients; **** p < 0.0001; ns indicates no significance. ( E ) Severity classification related to gender in the age range 30–65 years in the cohort of GB patients; **** p < 0.0001; ns indicates no significance.
Article Snippet: For fluorescent double-labeling of patient’s samples, goat anti-platelet-derived growth factor receptor beta (PDGFRβ; 1/250; BAF1042, R&D Systems, Barcelona, Spain), rabbit anti-LAMP-2A (1/1000; 51-2200, Invitrogen, Waltham, MA, USA) and mouse anti-RGS5 (1/150; MA5-25584, Invitrogen, Waltham, MA, USA) antibodies were used.
Techniques: Activity Assay, Expressing, Marker, Staining